E1385

Ethidiumbromid -Lösung

BioReagent, Molecular Biology, 500 μg/mL in H₂O

• Synonym(e): 3,8-Diamino-5-ethyl-6-phenyl-phenanthridiniumbromid, EtBr, Homidiumbromid
• Empirische Formel (Hill-System): C₂₁H₂₀BrN₃
• CAS-Nummer: 1239-45-8
• Molekulargewicht: 394.31
• UNSPSC Code: 41105322
• NACRES: NA.31
• PubChem Substance ID: 24894408
• MDL number: MFCD00011724
• Beilstein/REAXYS Number: 3642536

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioReagent
• concentration: 500 μg/mL in H₂O
• technique(s): electrophoresis: suitable
• suitability: suitable for gel electrophoresis
• SMILES string: [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• InChI key: ZMMJGEGLRURXTF-UHFFFAOYSA-N

Beschreibung

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy. It has also been used to detect DNA damage using comet assay.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

Für die Gelfärbung nach der Elektrophorese Stammlösung mit Wasser zu 0.5 μg/ml verdünnen und Gel für 15-30 min. inkubieren. Entfärben ist normalerweise nicht notwendig, kann aber gegebenenfalls für 15 min in Wasser durchgeführt werden um den Hintergrund zu veringern. Die DNA-Banden können dann in einer UV light box (Wellenlänge 254 nm) sichtbar gemacht werden. Es besteht auch die Option Ethidiumbromid in Gel und Laufpuffer zuzugeben (0.5 μg/ml), was eine Visualisierung unmittelbar nach der Elektrophorese ermöglicht.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable