HomeGas Chromatography (GC) GC Analysis of Glycerides in Coconut Oil (Organic, Extra Virgin) on SLB®-35ms
GC Analysis of Glycerides in Coconut Oil (Organic, Extra Virgin) on SLB®-35ms
Conditions | |
|---|---|
| Column | SLB-35ms, 10 m x 0.25 mm I.D., 0.10 µm; made by cutting down a 30 m x 0.25 mm I.D., 0.10 µm column (29802-U) |
| Oven | 75 °C (0.1 min), 15 °C/min to 380 °C (10 min) |
| Carrier gas | helium, 1.5 mL/min |
| Sample | coconut oil (organic, extra virgin), 0.02% (v/v) in chloroform |
| Injection | 1 µL, on-column |
| Inj. temp. | track oven: 75 °C (0.1 min), 15 °C/min to 380 °C (10 min) |
| Detector | FID, 380 °C |
Description | |
|---|---|
| Analysis Note | Triglycerides are the main constituent of vegetable oil and animal fat, and make up most of the fats digested by humans. Most natural fats contain a complex mixture of individual triglycerides. Therefore, efficient capillary GC columns are required for ample resolution and subsequent identification. Triglycerides are large compounds, and require a relatively high final oven temperature for GC analysis. For the analysis of triglycerides in extra virgin coconut oil, a short, thin film version of SLB-35ms (cut-down from a longer column) run to a final oven temperature of 380 ºC was found to provide good resolution and reasonable elution times. |
| Categories | Analytical Chromatography, GC Applications, Glycerides |
| Featured Industry | Agriculture Food and Beverages |
| Legal Information | SLB is a registered trademark of Sigma-Aldrich Co. LLC |
| Suitability | Application for GC |
Materials
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