Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improved separation of basic compounds
While the Ascentis® Express C8 and C18 provide classic reversed-phase selectivity, the RP-Amide phase provides alternate reversed-phase selectivity:
- Improved peak shape for basic compounds
- 100% aqueous compatibility
- LC-MS compatibility
- Fast separation with Fused-Core® technology
The Ascentis® Express RP-Amide HPLC column provides increased selectivity for polar compounds, especially those that can act as a hydrogen bond donor.
Ascentis® Express RP-Amide Specifications: | |
---|---|
Silica: | Type B (High purity silica) |
Particle Platform: | Superficially porous particles (SPP) |
Phase Chemistry: | C16-Amide |
Particle Size: | 2.0 µm / 2.7 µm / 5.0 µm |
Pore Size: | 90 Å |
Carbon Load: | 7.3% / 8.3% / 5.1% |
Surface Area: | 120 m²/g / 135 m²/g /90 m²/g |
pH Range: | 2 - 9 |
Max Temperature at Low pH: | 60 °C |
Max Temperature at High pH: | 40 °C |
Endcapped: | Yes |
Featured Application
RP-Amide phase with Fused-Core® particles for fast separation of basic compounds
Ascentis® Express RP-Amide is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design. The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:
2.0 µm particles
Best Fused-Core UHPLC Column
An optimized solution for high-throughput small molecule analysis
2.7 µm particles
Fast HPLC on ANY System
A practical solution that delivers UHPLC performance from any HPLC
5.0 µm particles
The Lab Work-Horse Column
True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns

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