Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

524629

Millipore

Phosphatase Inhibitor Cocktail V

50X, liquid, for the inhibition of serine/threonine and tyrosine phosphatase

Synonym(s):

Phosphatase Inhibitor Mix Set V, Phosphatase inhibitor

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

Phosphatase Inhibitor Cocktail Set V, 50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

shipped in

wet ice

storage temp.

−20°C

General description

A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.

Application

Phosphatase Inhibitor Cocktail Set V, 50X has been used:
  • as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
  • in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
  • in lysis buffer to lyse cultured HUVECs for immunoprecipitation
  • in wash buffer to wash the protein complexes
  • as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity

Biochem/physiol Actions

Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.

Features and Benefits

  • Convenient: formulations are ready-to-use, making them easy to use.
  • Consistent: high-quality formulations ensure reliable and reproducible performance.
  • Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.

Components

Each vial contains 1 ml of aqueous solution with the following components:
  • 250 mM Sodium Fluoride
  • 50 mM ß-Glycerophosphate (Cat. No. 35675)
  • 50 mM Sodium Pyrophosphate Decahydrate
  • 50 mM Sodium Orthovanadate      

Warning

Toxicity: Toxic (F)

Physical form

In H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Lysophosphatidylinositols in inflammation and macrophage activation: altered levels and anti-inflammatory effects
Masquelier J, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1863(12), 1458-1468 (2018)
Loss of transglutaminase 2 sensitizes for diet-induced obesity-related inflammation and insulin resistance due to enhanced macrophage c-Src signaling
Tibor S, et al.
Cell Death & Disease, 10(6), 439-439 (2019)
Umberto Tosi et al.
Science advances, 6(30), eabb4105-eabb4105 (2020-08-25)
Efforts at altering the dismal prognosis of pediatric midline gliomas focus on direct delivery strategies like convection-enhanced delivery (CED), where a cannula is implanted into tumor. Successful CED treatments require confirmation of tumor coverage, dosimetry, and longitudinal in vivo pharmacokinetic
Endothelial EphB4 maintains vascular integrity and transport function in adult heart
Guillermo L, et al.
eLife, 8, e45863-e45863 (2019)
Raphael Hesse et al.
Experimental neurology, 304, 1-13 (2018-02-22)
One major pathophysiological hallmark of Alzheimer's disease (AD) is senile plaques composed of amyloid β (Aβ). In the amyloidogenic pathway, cleavage of the amyloid precursor protein (APP) is shifted towards Aβ production and soluble APPβ (sAPPβ) levels. Aβ is known

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service