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Key Documents

CC145

Sigma-Aldrich

Rat Laminin

from rat bladder carcinoma, liquid, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

Rat Laminin-5,

biological source

rat

Quality Level

Assay

>95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

input

sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

General description

Laminin-5 is a basement membrane extracellular matrix macromolecule that provides an attachment substrate for both adhesion and migration in a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. It is a natural substrate for cells in contact with basement membranes, including epidermal and oral keratinocytes, gastro-intestinal tract, mammary and prostate epithelial cells. These cells, compared to fibronectin, collagen, or vitronectin, will adhere to Laminin-5 faster and spread to a larger extent. Laminin-5 can be used, in most applications, at coating concentrations in the 1 mg/ml or lower range, approximately 10-fold lower than most extracellular matrix macromolecules. Laminin-5 is attracting attention as a substrate that may stimulate carcinoma cell migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas. Receptors for Laminin-5 include integrins a3b1 and a6b4. The latter integrin is specialized in forming hemidesmosomes, which provide resistance to mechanical stress in many epithelia, including skin and gut. More recently, cell surface proteoglycans, such as syndecan-4, have been identified as receptors for Laminin-5. The structural and functional properties of Laminin-5 are well conserved across species. For instance, human cells adhere equally well to human and rat laminin-5. For most purposes, human and rat Laminin-5 are interchangeable, likely based on the fact that integrin receptor specificity is largely conserved across species.
Product Source: Rat bladder carcinoma cell line 804G {Koshikawa, N et al. (2000) J. Cell Biol. 148:615-624.

Application

Recommended coating concentrations: 1 μg/mL for cell adhesion assays, 0.2 μg/mL for cell migration assays.

Optimal dilutions must be determined by end user

Physical form

Purified protein supplied in 230mM tris, pH 7.5, 0.007% Brij-35, 0.1% CHAPS containing no preservatives.

Storage and Stability

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Variable

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5.
Giannelli, G, et al.
Science (New York, N.Y.), 277, 225-228 (1997)
The epithelium-tooth interface--a basal lamina rich in laminin-5 and lacking other known laminin isoforms.
Hormia, M, et al.
Journal of Dental Research, 77, 1479-1485 (1998)

Protocols

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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