EZHFGF21

Human FGF-21 ELISA Kit

measures FGF-21 in 50 μL serum, plasma or cell culture supernatent

• Synonym(s): ELISA Kit for FGF-21, FGF-21 ELISA, Human FGF-21 Detection Kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Properties

• product name: Human FGF-21 ELISA Kit, This Human FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
• Quality Level: 200
• species reactivity: human
• packaging: kit of 1 × 96 wells
• parameter: 50 μL sample volume (4hr assay)
• assay range: accuracy: 83-99%; standard curve range: 31.3-2000 pg/mL; inter-assay cv: <9%; intra-assay cv: <6%
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma (K2 EDTA); sample type cell culture supernatant
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production required by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. While insulin functions through GLUT4 translocation, FGF-21 has been shown to act primarily through the upregulation of adipocyte GLUT1, a transporter that allows glucose to move from the blood into cells even in the absence of insulin. In addition, no significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

Specificity

No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.

Application

Research Category
Metabolism

Research Sub Category
Diabetes - Type 2

Metabolic Syndrome

This Human FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.

This is a 3-hour assay requiring 50 μL of sample.

Used to detect/quantify: FGF-21

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS06,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H290,H302 + H332,H311,H317,H411
• Precautionary Statements: P234 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects