GF154

Wnt-3a, recombinant mouse

The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

• Synonym(s): Mouse Wnt-3a, Recombinant Wnt-3a
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• biological source: mouse
• Quality Level: 100
• mol wt: M_w 38.0-41.0 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 ng/μg endotoxin (1EU/μg)
• input: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s)
• UniProt accession no.: P27467
• shipped in: dry ice
• Gene Information: mouse ... WNT3A(22416)

Description

General description

Product Source: Mouse L cells expressing full length Wnt3a.

Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. When analyzed by SDS-PAGE under non-reducing conditions, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa.

Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. Wnt proteins have also been shown to have putative roles in the regulation of adult stem cells.

Application

Research Category
Stem Cell Research

The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

Linkage

Replaces: GF160

Physical form

Lyophilized from a solution containing PBS and 1.0% CHAPS buffer.

Storage and Stability

Maintain the lyophilized material at -20°C up to six months from date of receipt. Resuspend in sterile water to a concentration of 100µg/mL. Reconstituted Wnt3a should be stored in single use aliquots at -80°C for up to three months. Avoid repeated freeze/thaw cycles. During shipment, some amount of product will occasionally dispersed. It’s recommended to gently tape the vial on the hard surface before reconstitution.

Analysis Note

Specific Activity: Wnt3a protein has been shown to be bioactive as determined by TCF/LEF-luciferase reporter. The ED50 for this effect is typically <250 ng/mL. Optimal concentrations should be determined for each application.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2