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3022

Sigma-Aldrich

Transcreener® UDP2 TR-FRET Assay

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About This Item

UNSPSC Code:
41122100
NACRES:
NA.84

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® UDP2 TR-FRET Red Assay is a competitive immunoassay for UDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for UDP, the assay can be used with any enzyme that generates UDP from a UDP-sugar donor. Examples include glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetylgalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose, and hyaluronan synthases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® UDP2 TR-FRET Red Assay provides the following benefits:
  • Accommodates UDP-sugar donor concentrations ranging from 1 μM to 1,000 μM.
  • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
  • Overcomes the need for time-consuming, one-off assay development for individual members within an enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

Quantity

3022-1K = 1,000 assay, 384-well
3022-10K = 10,000 assay, 384-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Certificates of Analysis (COA)

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Drake Phelps et al.
Scientific reports, 7(1), 11244-11244 (2017-09-13)
Changes in resident microbiota may have wide-ranging effects on human health. We investigated whether early life microbial disruption alters neurodevelopment and behavior in larval zebrafish. Conventionally colonized, axenic, and axenic larvae colonized at 1 day post fertilization (dpf) were evaluated using

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