A3699

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 37.5:1

• Synonym(s): 30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution
• MDL number: MFCD00080848
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770843
• NACRES: NA.25

Properties

• product name: Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1
• sterility: 0.2 μm filtered
• Quality Level: 100
• product line: BioReagent
• form: liquid
• feed ratio: 37.5:1
• technique(s): electrophoresis: suitable
• suitability: suitable for electrophoresis
• storage temp.: 2-8°C; 2-8°C
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used:

• in the preparation of polyacrylamide gel for photobleaching experiment
• as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
• in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H332,H315,H317,H319,H340,H350,H361f,H372
• Precautionary Statements: P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves