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Key Documents

C4231

Sigma-Aldrich

Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal

clone BC-22, purified from hybridoma cell culture

Synonym(s):

Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BC-22, monoclonal

form

buffered aqueous solution

mol wt

antigen ~94 kDa

species reactivity

mouse, human, rat, chicken

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer33/pSer37)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

General description

CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Specificity

The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Application

Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for:
  • indirect ELISA
  • microarray
  • western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Biochem/physiol Actions

β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simon Megy et al.
FEBS letters, 580(22), 5411-5422 (2006-09-26)
The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer difference NMR (STD
Regulation of S33/S37 phosphorylated beta-catenin in normal and transformed cells.
Sadot E, et al.
Journal of Cell Science, 115(Pt 13), 2771-2780 (2002)
Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-KB pathway and mitotic checkpoint.
Uemura N, et al.
Cancer Science, 100(9), 1612-1622 (2009)
β-catenin is a valuable marker for differential diagnosis of osteoblastoma and osteosarcoma.
Wan Y, et al.
Human Pathology, 45(7), 1459-1465 (2014)
Activated macrophages promote Wnt/β-catenin signaling in cholangiocarcinoma cells.
Loilome W, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 35(6), 5357-5367 (2014)

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