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product name
PG-4, 94102703
biological source
feline brain
growth mode
Adherent
karyotype
Not specified
morphology
Glial/astrocyte
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Cat brain Moloney sarcoma virus-transformed
Cell Line Description
PG-4 was derived at NIH in 1980 from G355 cells by transformation with Moloney Murine sarcoma virus (M-MST) and subsequently cloned. Support the growth of simian sarcoma associated virus (SSAV); macaque virus; feline immunodeficiency virus (FeLV) A, B and C; feline RD-114; C retroviruses; murine xenotropic and munk cell focus-inducing virus (MCF).
Application
Cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.
PG-4 cells can be used to detect and assay type C retroviruses and are currently recommended by the FDA for the detection of replication competent retroviruses in gene therapy products and hybridomas producing reagents for human use.
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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