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Key Documents

G1794

Sigma-Aldrich

GAP-27

≥97% (HPLC)

Synonym(s):

Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile

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About This Item

Empirical Formula (Hill Notation):
C60H102N15O17
CAS Number:
198284-64-9
Molecular Weight:
1305.54
MDL number:
MFCD03456920
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

200

Assay

≥97% (HPLC)

form

powder

packaging

pkg of 2 mg

technique(s)

cell culture | mammalian: suitable

solubility

water: soluble 1.00-1.02 mg/mL, clear, colorless

UniProt accession no.

P35212

shipped in

ambient

storage temp.

−20°C

InChI

1S/C60H101N15O17/c1-9-31(4)44(54(86)69-41(29-36-19-13-12-14-20-36)52(84)70-45(32(5)10-2)55(87)72-46(59(91)92)33(6)11-3)71-57(89)48(35(8)78)73-51(83)38(21-15-16-26-61)66-50(82)39(24-25-43(79)80)67-56(88)47(34(7)77)74-53(85)42-23-18-28-75(42)58(90)40(22-17-27-65-60(63)64)68-49(81)37(62)30-76/h12-14,19-20,31-35,37-42,44-48,76-78H,9-11,15-18,21-30,61-62H2,1-8H3,(H,66,82)(H,67,88)(H,68,81)(H,69,86)(H,70,84)(H,71,89)(H,72,87)(H,73,83)(H,74,85)(H,79,80)(H,91,92)(H4,63,64,65)/t31-,32-,33-,34+,35+,37-,38-,39-,40-,41-,42-,44-,45-,46-,47-,48-/m0/s1

InChI key

SXRAPDIXXYFGJG-MDAHIHQXSA-N

Gene Information

human ... GJA4(2701)

Amino Acid Sequence

Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile

Application

GAP-27 has been used as a blocking peptide for Cx43 (connexin 43) gap junctions in FDC (follicular dendritic cells)-B cell cultures.

Biochem/physiol Actions

GAP-27 shares homology with a region of extracellular loops of connexins, and hence, is thought to inhibit junctions formed by the connexins Cx32, Cx40 and Cx43. It highly hinders co-operative cell-cell interactions including the synergized beating of embryonic cardiomyocytes. This peptide reduces osteoclastic activity and survival rates. This peptide significantly reduces acetylcholine-mediated hyperpolarization of smooth muscle cells.
Inhibitor of gap junction signaling in endothelial cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shaun L Sandow et al.
Circulation research, 90(10), 1108-1113 (2002-06-01)
The nature of the vasodilator endothelium-derived hyperpolarizing factor (EDHF) is controversial, putatively involving diffusible factors and/or electrotonic spread of hyperpolarization generated in the endothelium via myoendothelial gap junctions (MEGJs). In this study, we investigated the relationship between the existence of
Hajnalka Rajnai et al.
Journal of immunology research, 2015, 528098-528098 (2015-03-31)
Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested
J Ilvesaro et al.
BMC musculoskeletal disorders, 2, 10-10 (2001-12-19)
Bone remodelling is dependent on the balance between bone resorbing osteoclasts and bone forming osteoblasts. We have shown previously that osteoclasts contain gap-junctional protein connexin-43 and that a commonly used gap-junctional inhibitor, heptanol, can inhibit osteoclastic bone resorption. Since heptanol
Wenru Su et al.
Journal of immunology (Baltimore, Md. : 1950), 203(6), 1436-1446 (2019-08-20)
Therapeutic manipulation of regulatory T cells (Tregs) has been regarded as a promising approach for the treatment of immune disorders. However, a better understanding of the immunomodulatory mechanisms of Tregs and new safe and effective methods to improve the therapeutic

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