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I1645

Sigma-Aldrich

Interleukin-8 human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-8

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

mol wt

8.4 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL8(3576)

Application

Interleukin-8 (IL-8) human has ben used to:
  • measure neutrophil chemotoxis
  • study the uptake of apoptotic cells by meningothelial cells
  • study the effect of IL-8 on expression and secretion of urocortin-1 by human umbilical vein endothelial cells

Biochem/physiol Actions

Interleukin-8 (IL-8) was formerly called monocyte-derived neutrophil chemotactic factor. It belongs to the chemokine α or C-X-C family. Like other members of this family, the mature form of IL-8 has four cysteine residues and the first two cysteine residues are separated by glutamine. IL-8 shows chemotactic activity in vitro for T cells, neutrophils and basophils. Mutation in IL-8 can increase risk to acute pancreatitis.
Interleukin-8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the chemokine α or C-X-C family. The mature form of IL-8 has 4 cysteine residues, as do the other members of the chemokine family and the first two cysteine residues are separated by glutamine. Mature human IL-8 consists of 72 amino acids with a molecular mass of 8.4 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils, as measured by enzymes including myeloperoxidase, α-mannosidase and β-glucuronidase. IL-8 is an angiogenic factor.

Physical form

Lyophilized from a 0.2 μm filtered solution with no additives.

Analysis Note

The biological activity is determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ascentia M Seboko et al.
Frontiers in physiology, 9, 1812-1812 (2019-01-12)
It is known that advanced metabolic disorders such as type 2 diabetes compromise the functional and regenerative capacity of endogenous adipose-tissue resident stem cells (ADSCs). It is, however, still unclear at which stage of disease progression ADSCs become compromised and
X B Bao et al.
Genetics and molecular research : GMR, 14(1), 1508-1514 (2015-03-03)
We conducted a case-control study to clarify the asso-ciations between inflammatory cytokine, including interleukin (IL)-1b, IL-6, IL-8, and IL-10, polymorphisms and risk of acute pancreatitis. Genotyping analyses of IL-1β+3954 C/T (rs1143634), IL-1β-511 C/T (rs16944), IL-6 -174 G/C (rs1800795), IL-6 -634
D E Graugnard et al.
Journal of dairy science, 95(4), 1749-1758 (2012-03-31)
Cows experience some degree of negative energy balance and immunosuppression around parturition, making them vulnerable to metabolic and infectious diseases. The effect of prepartum feeding of diets to meet (control, 1.34 Mcal/kg of dry matter) or exceed (overfed, 1.62 Mcal/kg
Hyesol Lim et al.
Journal of cellular physiology, 236(10), 7014-7032 (2021-03-23)
Cancer-associated fibroblasts (CAFs) in the tumor microenvironment have been associated with tumor progression in breast cancer. Although crosstalk between breast cancer cells and CAFs has been studied, the effect of CAFs on non-neoplastic breast epithelial cells is not fully understood
Eugene L Bek et al.
Clinical science (London, England : 1979), 103 Suppl 48, 424S-429S (2002-08-24)
In diabetes mellitus, there is a problem of both premature atherosclerosis as well as impaired collateralization. Studies were performed using the rat corneal angiogenesis model as a surrogate for collateralization to determine the effect of diabetes mellitus on endothelin (ET)-1

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