P5400

Pectinesterase from orange peel

lyophilized powder, ≥150 units/mg protein

• Synonym(s): Pectin methylesterase, Pectin pectylhydrolase
• CAS Number: 9025-98-3
• Enzyme Commission number: 3.1.1.11 (BRENDA, IUBMB)
• EC Number: 232-807-0
• MDL number: MFCD00131812
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• specific activity: ≥150 units/mg protein
• composition: Protein, 20-50% biuret
• greener alternative product characteristics: Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: , Enabling
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH₄)₂SO₄ and sodium chloride. It has been used to study the pectin methylesterase gene.

The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Biochem/physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Unit Definition

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Physical form

Contains (NH₄)₂SO₄ and sodium chloride

Analysis Note

Protein determined by biuret.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves