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Key Documents

SAB4200723

Sigma-Aldrich

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal

clone WH-3, purified from hybridoma cell culture

Synonym(s):

Anti-TPH, Anti-TpOH

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

WH-3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

mouse, human, rabbit, sheep, rat

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using rabbit pineal gland lysate
immunofluorescence: suitable
immunohistochemistry: suitable

isotype

IgG3

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant rabbit Tryptophan Hydroxylase. Tryptophan Hydroxylase (TPH), also known as TpOH, TrpH or L-tryptophan tetrahydropteridine: oxygen oxidoreductase (EC 1.14.16.4) is a member of biopterin-dependent aromatic amino acid hydroxylases family, together with tyrosine hydroxylase and phenylalanine hydroxylase. This gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. This 444 amino acid protein is expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.

Immunogen

recombinant rabbit Tryptophan Hydroxylase.

Application

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

Tryptophan Hydroxylase (TPH) is a rate-limiting tetramer enzyme in the synthetic pathway of neurotransmitter serotonin from L-tryptophan and is considered a key factor for the maintenance of normal serotonin transmission in the central nervous system. The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders.The encoded protein plays a vital role in regulation of cardiovascular function.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TPH1 A218C polymorphism and temperament in major depression
Andre K, et al.
BMC Psychiatry, 13(1), 118-118 (2013)
The tryptophan hydroxylase 1 (TPH1) gene, schizophrenia susceptibility, and suicidal behavior: A multi-centre case-control study and meta-analysis
Saetre P, et al.
American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics, 153(2), 387-396 (2010)
Tryptophan hydroxylase 1 gene polymorphisms alter prefrontal cortex activation during response inhibition.
Ruocco AC, et al.
Neuropsychology, 30(1), 18-18 (2016)
Characterization of a stable form of tryptophan hydroxylase from the human parasite Schistosoma mansoni
Hamdan FF and Ribeiro P
The Journal of Biological Chemistry, 274(31), 21746-21754 (1999)
Disruption of the nonneuronal tph1 gene demonstrates the importance of peripheral serotonin in cardiac function
Cote F, et al.
Proceedings of the National Academy of Sciences of the USA, 100(23), 13525-13530 (2003)

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