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V0882

Sigma-Aldrich

D-Val-Leu-Lys 4-nitroanilide dihydrochloride

plasmin substrate

Synonym(s):

D-Val-Leu-Lys-pNA dihydrochloride, D-Valyl-L-leucyl-L-lysine 4-nitroanilide dihydrochloride

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About This Item

Empirical Formula (Hill Notation):
C23H38N6O5 · 2HCl
CAS Number:
62354-43-2
Molecular Weight:
551.51
MDL number:
MFCD00077899
UNSPSC Code:
12352204
PubChem Substance ID:
24900701
NACRES:
NA.32

Assay

≥98% (TLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Cl.Cl.CC(C)C[C@H](NC(=O)[C@H](N)C(C)C)C(=O)N[C@@H](CCCCN)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C23H38N6O5.2ClH/c1-14(2)13-19(28-23(32)20(25)15(3)4)22(31)27-18(7-5-6-12-24)21(30)26-16-8-10-17(11-9-16)29(33)34;;/h8-11,14-15,18-20H,5-7,12-13,24-25H2,1-4H3,(H,26,30)(H,27,31)(H,28,32);2*1H/t18-,19-,20+;;/m0../s1

InChI key

VESQMNNSPPEOSZ-ZLARAOTRSA-N

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Application

D-Val-Leu-Lys 4-nitroanilide dihydrochloride has been used as a chromogenic substrate to determine the formation of plasmin from plasminogen in amidolytic activity assay and plasminogen activating assay.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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S Ueshima et al.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 7(5), 522-529 (1996-07-01)
Staphylokinase (SAK), produced by Staphylococcus aureus, induces fibrinolytic activity in circulation without systemic fibrinolytic activation. Since the effect of blood vessels on the activity of SAK has not yet been clarified, plasminogen activator (PA) activity of SAK in the presence
Sahin Sultana et al.
Immunology letters, 209, 53-66 (2019-03-30)
uPA/tPA-mediated activation of plasminogen/plasmin pathway during S. aureus arthritis facilitates the invasion of phagocytes in the affected joint, induces production of cytokines and triggers inflammatory pathways. PAI-1, an effective inhibitor of both uPA and tPA, attenuates plasmin activity. Hence, the
L C Petersen
European journal of biochemistry, 245(2), 316-323 (1997-04-15)
The two zymogens, plasminogen and pro-urokinase plasminogen activator (pro-uPA), constitute a system of reciprocal activation, since plasmin, generated by uPA-catalysed plasminogen activation, can activate pro-uPA to uPA. Two such zymogens, when mixed, will undergo autocatalytic, reciprocal activation resulting in generation
Genetic modification of the streptokinase gene to study the interaction of streptokinase with plasminogen.
S Lizano et al.
Developments in biological standardization, 85, 195-198 (1995-01-01)
I A Taitzoglou et al.
Reproduction (Cambridge, England), 121(1), 131-137 (2001-02-28)
The effect of tannic acid, a common flavonoid, on the acrosin and plasminogen activator activity and plasmin activity of human and ram spermatozoa was evaluated. Acrosin and plasminogen activator activity were determined by spectrophotometry using the chromogenic substrates N-alpha-benzoyl-DL-arginine para-nitroanilide-HCl
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Protocols

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

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