ZMS1057

Anti-Cytokeratin 5/6 Antibody, clone D5/16 B4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: D5/16 B4, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 62.38 kDa; observed mol wt ~62 and 55 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1
• epitope sequence: Unknown
• Protein ID accession no.: NP_000415.2
• UniProt accession no.: P13647
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone D5/16 B4 is a a ZooMAb^® mouse recombinant monoclonal antibody that detects human cytokeratins 5 and 6.

Immunogen

Purified human Cytokeratin 5.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Cytokeratin 5/6 in human tonsil tissue sections.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytokeratin 5/6 in human skin tissue sections.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cytokeratin 5/6 in A431 and HaCaT cell lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Cytokeratin 5/6 in human skin tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Cytokeratin 5/6, clone D5/16 B4 ZooMAb^®, Cat. No. ZMS1057, is a Mouse recombinant antibody that detects Cytokeratin 5/6 and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Keratin, type II cytoskeletal 5 (UniProt: P13647; also known as 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5) is encoded by the KRT5 gene (Gene ID: 3852) in human. Keratins are the intermediate filament proteins of epithelia that display high degree of molecular diversity. They are heteropolymeric filaments formed by pairing of type I and type II keratins that are expressed in a highly specific patterns depending on the epithelial type and stage of cellular differentiation. Keratins contain a central rod domain of about 310 amino acids with alpha-helical conformation bordered by non-helical head and tail domains of variable length. The head domain consists of subdomains V1 and H1. The central alpha helical rod domain is composed of subdomains 1A, 1B, 2A, and 2B connected by the linkers L1, L12, and L2. The tail domain then consists of subdomains H2 and V2. They provide stability between epithelial cells and their attachment to basement membrane. Keratins also serve as tumor markers and epithelial differentiation markers. Multiple human epithelial keratins have been resolved with 2D-gels electrophoresis. These can be divided into acidic (pI 5.7) and basic (pI 6.0) subfamilies. The acidic keratins have molecular weights that range from 40 to 56 kDa. The basic keratins have molecular weights that range from 52 to 67 kDa. Members of the acidic and basic subfamilies are reported to be present in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and the disease state. Cytokeratins 5 and 6 are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma and are shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung. Clone D5/16 B4 recognizes cytokeratin 5 and 6. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1