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Lipofection reagents prepared by a simple ethanol injection technique.

BioTechniques (1995-06-01)
M J Campbell
ABSTRACT

Cationic liposomes are being utilized for the delivery of DNA into mammalian cells both in vitro and in vivo. In this report, we describe a rapid, simple injection method for the preparation of cationic liposomes that requires no special equipment. We demonstrate that, using this injection method, liposomes prepared with a commercially available cationic lipid (dimethyldioctadecylammonium bromide, DDAB) or a synthetic cationic cholesterol derivative, along with a neutral lipid (dioleoyl-L-alpha-phosphatidylethanolamine, DOPE), effectively transfect a variety of cell lines in vitro. The transfection activity of liposomes prepared by this method was comparable to that obtained with liposomes prepared by the standard evaporation/sonication procedure and to that of a commercial reagent, Lipofectamine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cholesteryl 3β-N-(di­methyl­amino­ethyl)­carbamate hydrochloride, ≥95%
Sigma-Aldrich
L-α-Phosphatidylethanolamine, dioleoyl, ≥99% (GC), ≥98% (TLC), lyophilized powder