- Specific fluorescence labeling of target proteins by using a ligand-4-azidophthalimide conjugate.
Specific fluorescence labeling of target proteins by using a ligand-4-azidophthalimide conjugate.
Chemical communications (Cambridge, England) (2017-07-21)
Kosuke Chiba, Miwako Asanuma, Minoru Ishikawa, Yuichi Hashimoto, Kosuke Dodo, Mikiko Sodeoka, Takao Yamaguchi
PMID28726872
ABSTRACT
We herein propose a simple affinity-labeling method using a ligand-4-azidophthalimide (AzPI) conjugate. As a proof of concept, we show that two different ligand-AzPI conjugates enabled highly specific fluorescence labeling of their individual target proteins even in crude cell lysates. This method was also applied to label endogenous target proteins inside living cells.
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