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  • Vitamin A is systemically bioavailable after intratracheal administration with surfactant in an animal model of newborn respiratory distress.

Vitamin A is systemically bioavailable after intratracheal administration with surfactant in an animal model of newborn respiratory distress.

Pediatric research (2010-03-11)
Avash J Singh, Vadim Bronshtein, Minesh Khashu, Kyle Lee, James E Potts, James Friel, Philippe Chessex
ABSTRACT

Chronic lung disease (CLD) is a major cause of long-term morbidity in extremely LBW infants with respiratory distress syndrome. Parenteral vitamin A administration decreases the risk of CLD. We tested the hypothesis that intratracheal vitamin A administration with surfactant is systemically bioavailable without interfering with the functional properties of exogenous surfactant. Newborn piglets were ventilated with 100% FiO2 and sequential saline lavage induced respiratory distress syndrome. During lung injury induction, ventilator changes were allowed, but none were made following treatment allocation. Animals were assigned by chance in a blinded control trial to three groups: I=control; II=surfactant; III=surfactant+vitamin A. Hemodynamics, lung mechanics, and blood gases were measured following instrumentation, pre- and posttreatment for 4 h, at which time the liver was sampled for retinol determination. All parameters improved in animals receiving surfactant. A significant interaction existed between time and group for PaO2 and alveolar-arterial oxygen difference (A-aDO2). Hepatic levels of retinol were higher (p<0.001) in animals receiving retinyl acetate. Intratracheal administration of surfactant+vitamin A did not alter the beneficial effects of surfactant on lung compliance and gas exchange. Intratracheal Vitamin A was associated with rapid hepatic uptake. Further studies are warranted.

MATERIALS
Product Number
Brand
Product Description

Supelco
Retinyl Acetate (Vitamin A Acetate), Pharmaceutical Secondary Standard; Certified Reference Material
Retinol acetate, European Pharmacopoeia (EP) Reference Standard
Supelco
Retinyl acetate, analytical standard
Sigma-Aldrich
Retinyl acetate, synthetic, matrix dispersion, 475,000-650,000 USP units/g
Sigma-Aldrich
Retinyl acetate, synthetic, crystalline solid or supercooled liquid
Sigma-Aldrich
Retinyl acetate, BioReagent, solid or viscous liquid, synthetic, suitable for cell culture