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  • Template-Assisted Plasmonic Nanogap Shells for Highly Enhanced Detection of Cancer Biomarkers.

Template-Assisted Plasmonic Nanogap Shells for Highly Enhanced Detection of Cancer Biomarkers.

International journal of molecular sciences (2021-02-14)
Homan Kang, Sinyoung Jeong, Jin-Kyoung Yang, Ahla Jo, Hyunmi Lee, Eun Hae Heo, Dae Hong Jeong, Bong-Hyun Jun, Hyejin Chang, Yoon-Sik Lee
ABSTRACT

We present a template-assisted method for synthesizing nanogap shell structures for biomolecular detections based on surface-enhanced Raman scattering. The interior nanogap-containing a silver shell structure, referred to as a silver nanogap shell (Ag NGS), was fabricated on silver nanoparticles (Ag NPs)-coated silica, by adsorbing small aromatic thiol molecules on the Ag NPs. The Ag NGSs showed a high enhancement factor and good signal uniformity, using 785-nm excitation. We performed in vitro immunoassays using a prostate-specific antigen as a model cancer biomarker with a detection limit of 2 pg/mL. To demonstrate the versatility of Ag NGS nanoprobes, extracellular duplex surface-enhanced Raman scattering (SERS) imaging was also performed to evaluate the co-expression of cancer biomarkers, human epidermal growth factor-2 (HER2) and epidermal growth factor receptor (EGFR), in a non-small cell lung cancer cell line (H522). Developing highly sensitive Ag NGS nanoprobes that enable multiplex biomolecular detection and imaging can open up new possibilities for point-of-care diagnostics and provide appropriate treatment options and prognosis.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
4-Chlorothiophenol, 97%
Sigma-Aldrich
4-Bromothiophenol, 95%
Sigma-Aldrich
4-Fluorothiophenol, 98%