Skip to Content
MilliporeSigma
  • Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells.

Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells.

International journal of molecular sciences (2022-02-16)
Gae-Baik Kim, Jeong Min Lee, Duc Long Nguyen, Joonseok Lee, Young-Pil Kim
ABSTRACT

Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MMP-9, Active, Human, Recombinant
Sigma-Aldrich
MMP-2, Active, Human, Recombinant, Mouse Cells