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  • Critical evaluation of comparative model building of Streptomyces griseus trypsin.

Critical evaluation of comparative model building of Streptomyces griseus trypsin.

Biochemistry (1984-12-18)
R J Read, G D Brayer, L Jurásek, M N James
ABSTRACT

The X-ray crystal structure of Streptomyces griseus trypsin has been solved and refined at 1.7-A resolution. The structure of this protein had been predicted in two models on the basis of its expected homology to structures of bovine trypsin and other pancreatic serine proteases [Jurásek, L., Olafson, R.W., Johnson, P., & Smillie, L.B. (1976) Miami Winter Symp. 11, 93-123; Greer, J. (1981) J. Mol. Biol. 153, 1027-1042]. An evaluation of these models in light of the known structure demonstrates the effect of several sources of error on such comparative model building. The objective of comparative model building is often to explain substrate specificity, or to suggest potential highly specific drugs. The unique parts of modeled proteins that are most important for such purposes are, however, the most poorly determined by the model-building procedure.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid
Sigma-Aldrich
Trypsin from bovine pancreas, essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin from bovine pancreas, Type I, ~10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, ≥2,500 USP units/mg solid, meets USP testing specifications
Sigma-Aldrich
Trypsin from bovine pancreas, suitable for protein sequencing, lyophilized powder
Sigma-Aldrich
Trypsin Acetylated from bovine pancreas, Type V-S, ≥8,500 BAEE units/mg protein (biuret)
Sigma-Aldrich
Trypsin from bovine pancreas, Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
Trypsin from human pancreas, salt-free, lyophilized powder, vial of ≥1,000 BAEE units
Sigma-Aldrich
Trypsin from porcine pancreas, ~1500 U/mg
Sigma-Aldrich
TrypZean® bovine, recombinant, expressed in corn, lyophilized powder, ≥3,350 units/mg solid (USP)
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid
Sigma-Aldrich
Trypsin from porcine pancreas, tablet, 1 mg tablet
Sigma-Aldrich
Trypsin from porcine pancreas, lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid
Sigma-Aldrich
Trypsin from porcine pancreas, Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein