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A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

Organic & biomolecular chemistry (2014-12-17)

S Bag, J-C Tseng, J Rochford

ABSTRACT

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

VenPure^® SF, powder

Sigma-Aldrich

Triethylamine, ≥99%

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Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder

Supelco

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Tetrahydrofuran, suitable for HPLC, ≥99.9%, inhibitor-free

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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

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Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)

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2,3-Dichloro-5,6-dicyano-p-benzoquinone, 98%

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Sodium borohydride solution, ~12 wt. % in 14 M NaOH

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Boron, crystalline, −60 mesh, 99% trace metals basis

Supelco

Triethylamine, analytical standard

Boron, monofilament, 20m, diameter 0.1mm

Boron, monofilament, 50m, diameter 0.1mm

Boron, monofilament, 10m, diameter 0.2mm

Boron, monofilament, 200m, diameter 0.1mm

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Tetrahydrofuran, Pharmaceutical Secondary Standard; Certified Reference Material

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Boron, monofilament, 10m, diameter 0.1mm

Boron, monofilament, 5m, diameter 0.1mm

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Sigma-Aldrich

Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

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Triethylamine, ≥99.5%

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Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

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Triethylamine, puriss. p.a., ≥99.5% (GC)

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