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  • Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells.

Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells.

Biomaterials (2014-05-09)
Li Liu, Momoko Yoshioka, Minako Nakajima, Arata Ogasawara, Jun Liu, Kouichi Hasegawa, Sisi Li, Jianli Zou, Norio Nakatsuji, Ken-Ichiro Kamei, Yong Chen
ABSTRACT

Nanofibrous gelatin substrates are suited for long-term expansion of human pluripotent stem cells (hPSCs) under feeder- and serum-free culture conditions. A combinatorial library with different sets of processing parameters was established to assess the culture performance of hPSCs on nanofibrous substrates in terms of cell adhesion and growth rate, using Matrigel as control. Then, the optimal conditions were applied to long-term expansion of hPSCs with several cell lines, showing a maintained pluripotency over more than 20 passages without introducing any abnormal chromosome. In addition, this approach allowed us to avoid enzymatic disassociation and mechanic cutting during passages, thereby promoting a better hPSC culture and long-term expansion.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
FGF-2 human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Sigma-Aldrich
FGF-2 human, recombinant, expressed in insect cells, ≥85% (SDS-PAGE)