HPLC Analysis of Immunoglobulins (IgM) on Q Sepharose^™ Fast Flow

media

Q Sepharose Fast Flow (Q1126-100ML)

column

30 cm × 50 mm I.D. (Q1126-100ML)

mobile phase

[A] 0.02 M monosodium phosphate, pH 6.5; [B] A + 0.5 M sodium chloride, pH 6.5

flow rate

25 cm/hr

detector

UV, 280 nm

injection

cell culture supernatant