HPLC Analysis of Phenolic Compounds on Ascentis® Express 2.7 μm RP-Amide
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Resorcinol
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandHydroquinone
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase
[A] 20 mM phosphoric acid, pH 2; [B] acetonitrile; (75:25, A:B)
flow rate
1.5 mL/min
column temp.
35 °C
detector
UV, 270 nm
injection
25 μL
sample
50 mg/L in mobile phase
Description
Analysis Note
Chromatography of Seven Phenol Compounds on Ascentis Express RPA
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany