HPLC Analysis of Thalidomide Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 18 mM ammonium nitrate, pH 5.5; [B] acetonitrile; (65:35, A:B)

flow rate

1 mL/min

column temp.

22 °C

detector

UV, 240 nm

injection

20 μL

sample

1 mg/mL in methanol