HPLC Analysis of Triacylglycerols in Olive Oil on Discovery^® HS C18 after Sample Prep using Discovery^® DSC-Si (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

SPE tube/cartridge

Discovery DSC-Si, 1 gm/6 mL (52656-U)

condition

6 mL hexane

sample addition

0.12 g oil in 0.5 mL hexane

elution

10 mL hexane-diethyl ether 87-13 (v/v)

eluate post-treatment

evaporation to dryness, reconstituted into 2 mL acetone

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

[A] acetone; [B] acetonitrile; (60:40, A:B)

flow rate

1.5 mL/min

pressure

914 psi (63 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL