HPLC Analysis of Yohimbe Alkaloids on Ascentis^® Express F5, HILIC Mode

column

Ascentis Express F5, 3 cm x 3.0 mm I.D., 2.7 μm particles (53574-U)

mobile phase

2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 6.0 with acetic acid

flow rate

0.5 mL/min

pressure

1100 psi (76 bar)

column temp.

35 °C

detector

UV, 275 nm

injection

2 μL

sample

100 ug/mL each in methanol