QR0200

Quantitative RT-PCR ReadyMix^™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

• Synonyme(s) : 1-step RT-qPCR mix, Quantitative real-time PCR master mix
• NACRES: NA.55
• UNSPSC Code: 41106300

Propriétés

• Quality Segment: 300
• usage: sufficient for 250 reactions
• feature: dNTPs included, hotstart
• technique(s): RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable
• color: colorless
• input: purified RNA
• compatibility: ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf^® Mastercycler ep realplex2 s, Eppendorf^® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000
• detection method: probe-based
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Quantitative RT-PCR ReadyMix^™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix^™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot Start methods
• JumpStart^™Taq Polymerase reduces primer-dimer and non-specific product formation
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Legal Information

This product is for research use only.

Eppendorf is a registered trademark of Eppendorf SE

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

• Probe Based qRT-PCR ReadyMix 2 X
• Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

Composants de kit également disponibles séparément

• P2192

  10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1.5 mL/vial

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• M8787

  Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 25 mM

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• P2192

  10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 100 X

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Informations sur la sécurité

• Pictogrammes: GHS07,GHS09
• Classe de stockage: 12 - Non Combustible Liquids
• Que se passe-t-il ?: WGK 3
• Mot-clé: Danger
• Codes de danger: H315,H317,H319,H410,EUH430
• Mentions de précaution: P201 - P202 - P273 - P280 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1