G1N70

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 70 purifications

• Synonyma: Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
• NACRES: NA.52
• UNSPSC Code: 41105501

Vlastnosti

• usage: sufficient for 70 purifications
• Quality Segment: 200
• technique(s): DNA purification: suitable
• input: mammalian cells; mammalian tissue
• test parameters: sample volume: 25 mg tissue, sample volume: 2x10^6 cells
• storage temp.: 15-25°C

Popis

General description

Click here to view the kit protocol

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Biochem/physiol Actions

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Součásti soupravy, které jsou dostupné také samostatně

• P2308

  Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3

  Bezpečností list
• R6148

  RNase A solution

  Bezpečností list
• C2112

  Column Preparation Solution

  Bezpečností list

Bezpečnostní informace

• Piktogramy: GHS08,GHS05,GHS07,GHS09
• Výstražné slovo: Danger
• Cílové orgány: Respiratory system
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• Co se děje?: WGK 3
• Kódy nebezpečí: H290,H302,H315,H318,H334,H335,H410,EUH430
• Bezpečnostní pokyny: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Skladovací třída: 10 - Combustible liquids