NA1020

GenElute^™ PCR Clean-Up Kit

sufficient for 70 purifications

• Synonyma: Gen Elute, PCR Purification
• NACRES: NA.52
• UNSPSC Code: 41106300

Vlastnosti

• usage: sufficient for 70 purifications
• Quality Segment: 200
• technique(s): DNA purification: suitable
• input: DNA (single or double stranded PCR ampliflied)
• test parameters: sample volume: 10 μg, sample volume: 100 μL
• storage temp.: 15-25°C

Popis

General description

Click here to view the kit protocol

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.

Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Application

Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

GenElute^™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.

Biochem/physiol Actions

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Features and Benefits

• Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
• Recovers up to 95% of PCR products between 100 bp and 10 kb
• Removes over 99% of primers and other components
• Eliminates the need to remove mineral oil by organic extraction
• 40% more purification preps supplied than market leader

• Recovers up to 95% of PCR products between 100 bp and 10 kb
• Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
• Removes over 99% of primers and other components

Other Notes

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Součásti soupravy, které jsou dostupné také samostatně

• C2112

  Column Preparation Solution

  Bezpečností list

Bezpečnostní informace

• Piktogramy: GHS05,GHS07
• Výstražné slovo: Warning
• Kódy nebezpečí: H290,H302,H315,H319,H336
• Bezpečnostní pokyny: P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Cílové orgány: Central nervous system
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• Co se děje?: WGK 2
• Skladovací třída: 10 - Combustible liquids