SHC204

MISSION^® TRC2 pLKO.5-puro TurboGFP^™ shRNA Control Plasmid DNA

shRNA sequence targeting tGFP

• Synonym(s): MISSION^® Control Vectors
• UNSPSC Code: 41106609
• NACRES: NA.51
• MDL number: MFCD07785395
• Concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Properties

• Quality Segment: 100
• product line: MISSION^®
• concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The MISSION^® TRC2 Control Vector pLKO-puro TurboGFP^™ shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP^™. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP^™ shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION^® TurboGFP^™ positive control vector in cell lines expressing TurboGFP^™. TurboGFP^™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP^™ shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens.

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable