SRP3174
TIMP-2 human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonym(s):
CSC-21K, Tissue inhibitor of metalloproteinase 2
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
potency
0.5 μg/mL
mol wt
21.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TIMP2(7077)
General description
Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs) including MMP1, 2, 3, 7, 8, 9, 10, 12, 13, 14, 15, 16 and 19. It belongs to the I35 (TIMP) family of irreversible protease inhibitors. The gene encoding this protein is localized on human chromosome 17q25. Recombinant human TIMP-2 is a 21.8kDa protein containing 194 amino acid residues.
Biochem/physiol Actions
Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22.
Sequence
MCSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Growth-stimulatory activity of TIMP-2 is mediated through c-Src activation followed by activation of FAK, PI3-kinase/AKT, and ERK1/2 independent of MMP inhibition in lung adenocarcinoma cells.
Kim Hle
Oncotarget, 6(40), 42905-42922 (2015)
D E Gomez et al.
European journal of cell biology, 74(2), 111-122 (1997-11-14)
Four members of the tissue inhibitor of metalloproteinases (TIMP) family have been characterized so far, designated as TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 and TIMP-2 are capable of inhibiting the activities of all known matrix metalloproteinases (MMPs) and as such
Genetics of Moyamoya disease.
Roder C
Journal of Human Genetics, 55(11), 711-716 (2010)
Hui Wang et al.
PloS one, 9(5), e96982-e96982 (2014-05-09)
Vasculogenic mimicry (VM) is a newly-defined tumor microcirculation pattern in highly aggressive malignant tumors. We recently reported tumor growth and VM formation of gallbladder cancers through the contribution of the ephrin type a receptor 2 (EphA2)/focal adhesion kinase (FAK)/Paxillin signaling
Promotion of astrocytoma cell invasion by micro RNA-22 targeting of tissue inhibitor of matrix metalloproteinase-2.
Ohnishi YI
Journal of Neurosurgery. Spine, 26(3), 396-403 (2017)
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