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  • Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC).

Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC).

STAR protocols (2022-09-14)
Manuela Sauter, Reinhard J Sauter, Henry Nording, Marcus Olbrich, Frederic Emschermann, Harald F Langer
ABSTRACT

Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c+ cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021).

MATERIALS
Product Number
Brand
Product Description

Roche
Streptavidin-AP conjugate, solution, pkg of 1 mL (1,000U)
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SKUPack SizeAvailabilityPriceQuantity
500 mg
1 Available to ship on 16 December 2024
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€135.00