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Sugar metabolism in relation to chilling tolerance of loquat fruit.

Food chemistry (2012-09-29)
Shifeng Cao, Zhenfeng Yang, Yonghua Zheng
ABSTRACT

The relationship between chilling injury and sugar metabolism was investigated in loquat fruit stored at 1°C for 35days. No symptoms of chilling injury occurred in the fruit, of 'Ninghaibai' cultivar, during the whole storage whereas, in 'Dahongpao' fruit, severe chilling symptoms were observed after 20days of storage at 1°C. 'Ninghaibai' fruit had higher levels of glucose and fructose and higher activities of sucrose hydrolyzing enzymes, such as sucrose synthase-cleavage and invertase, than had 'Dahongpao'. Furthermore, the chilling resistant 'Ninghaibai' fruit also showed higher activities of hexokinase and fructokinase, involved in hexose phoshorylation and sugar signal generation. These results suggest that the higher content of hexoses and activities of hexose sensors were likely part of the mechanism for chilling tolerance of loquat fruit.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Invertase from Candida utilis, Grade X, ≥300 units/mg solid
Sigma-Aldrich
Invertase from baker′s yeast (S. cerevisiae), Grade VII, ≥300 units/mg solid
Supelco
Invertase from baker′s yeast (S. cerevisiae), 200-300 units/mg solid
Sigma-Aldrich
Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomics