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AEC101

Sigma-Aldrich

AEC Staining Kit

liquid

Synonym(s):

ACE substrate kit

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.32

Pricing and availability is not currently available.

form

liquid

Quality Level

usage

sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.

2-8°C

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Lead foil, light tested, 25x25mm, thickness 0.007mm, 99.99+%

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Lead foil, light tested, 25x25mm, thickness 0.025mm, 99.99+%

GF15751122

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Lead foil, light tested, 25x25mm, thickness 0.05mm, 99.99+%

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Lead foil, light tested, 25x25mm, thickness 0.02mm, 99.99+%

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Lead

manufacturer/tradename

Goodfellow 753-560-95

manufacturer/tradename

Goodfellow 157-511-22

manufacturer/tradename

Goodfellow 080-566-49

manufacturer/tradename

Goodfellow 174-939-69

assay

99.99%

assay

99.99%

assay

99.99%

assay

99.99%

resistivity

20.65 μΩ-cm

resistivity

20.65 μΩ-cm

resistivity

20.65 μΩ-cm

resistivity

20.65 μΩ-cm

mp

327.4 °C (lit.)

mp

327.4 °C (lit.)

mp

327.4 °C (lit.)

mp

327.4 °C (lit.)

bp

1740 °C (lit.)

bp

1740 °C (lit.)

bp

1740 °C (lit.)

bp

1740 °C (lit.)

Application

AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods[1]
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay[2]
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads[3]

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Caution

Stable at least one year at 2-8 °C.

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

134.6 °F - closed cup

Flash Point(C)

57 °C - closed cup


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    Protocols

    Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

    Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

    Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

    Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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