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Merck

N1531

Sigma-Aldrich

Denaturation Solution

for Northern and Southern blotting

Synonym(s):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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1 L
€60.20

€60.20


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1 L
€60.20

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

€60.20


Please contact Customer Service for Availability

grade

for molecular biology

Quality Level

form

solution

storage temp.

room temp

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This Item
D2532D5688P1415
form

solution

form

solution

form

liquid

form

liquid (2x concentrate)

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100, 200

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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José Manuel Pérez-Pérez et al.
Plant physiology, 134(1), 101-117 (2004-01-20)
The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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