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RPROB

Sigma-Aldrich

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

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100 μL
€481.00

€481.00


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100 μL
€481.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

€481.00


Please contact Customer Service for Availability

usage

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

Quality Level

technique(s)

protein staining: suitable

storage temp.

2-8°C

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This Item
5379744259931716
format

neat

format

neat

format

neat

format

neat

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

grade

analytical standard

grade

certified reference material, TraceCERT®

grade

analytical standard

grade

analytical standard

storage temp.

2-30°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

packaging

ampule of 10 mg

packaging

-

packaging

ampule of 50 mg

packaging

-

Application

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels[1] with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

Other Notes

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

Kit Components Only

Product No.
Description

  • Solution A 500 mL

  • Solution B 500 mL

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials


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    Find documentation for the products that you have recently purchased in the Document Library.

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    Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
    Rachel R.
    Electrophoresis (1997)

    Articles

    Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

    Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

    Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

    Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

    Protocols

    Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

    Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

    Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

    Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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