Cell Culture Antibiotic Selection Guide
Antibiotics are commonly used in cell culture to prevent contamination, maintain aseptic conditions, or select for cells containing genetic modifications. The use of antibiotics can minimize loss of cell lines and cell cultures, saving time and conserving precious resources. Proper antibiotic selection depends on both cell type and which contaminants you are trying to prevent. Selective antibiotics for generating stable cell lines or other recombinant cultures should be chosen based on the antibiotic resistance gene or selectable marker. The tables below can be used to guide antibiotic selection for your cell culture needs.
Antibiotic by Target
Cell Membrane Integrity
Protein Synthesis
Inhibit | Classification | Product Type | Product No. | Antibiotic Spectrum | Selection Antibiotic | |||
Gram -ve | Gram +ve | Fungi | Mycoplasma | |||||
30S ribosome subunit | Tetracyclines | Tetracycline | x | x |
|
| x | |
Doxycycline | x | x |
|
| x | |||
Aminocyclitol | Spectinomycin | x | x |
|
| x | ||
x | x |
|
| x | ||||
Hygromycin | x | x | x |
|
| |||
x | x | x |
|
| ||||
polypeptide synthesis | Aminoglycosides | Streptomycin | x | x |
|
|
| |
x | x |
|
|
| ||||
Paromomycin | x | x |
|
|
| |||
x | x |
|
|
| ||||
Gentamicin | x | x |
| x |
| |||
x | x |
| x |
| ||||
x | x |
| x |
| ||||
x | x |
| x |
| ||||
x | x |
| x |
| ||||
Kanamycin | x | x |
| x | x | |||
x | x |
| x | x | ||||
Neomycin | x | x |
|
| x | |||
x | x |
|
| x | ||||
x | x |
|
| x | ||||
G418 |
|
|
|
| x | |||
|
|
|
| x | ||||
|
|
|
| x | ||||
Nourseothricin | x | x | x |
| x | |||
50S ribosome subunit | Macrolides | Tylosin |
| x |
| x |
| |
| x |
| x |
| ||||
Erythromycin |
| x |
|
|
| |||
Nystatin |
|
| x |
|
| |||
|
| x |
|
| ||||
|
| x |
|
| ||||
|
| x |
|
| ||||
|
| x |
|
| ||||
Lincomycin |
| x |
|
|
| |||
Rifampicin |
| x |
|
| x | |||
peptidyl transferase | Chloramphenicols | Chloramphenicol | x | x |
| x | x |
Nucleic Acid Synthesis
Inhibit | Classification | Product Type | Product No. | Antibiotic Spectrum | Selection Antibiotic | ||
Gram -ve | Gram +ve | Cancer cells | |||||
DNA synthesis | IMPDH inhibitors | Mycophenolic acid |
|
| x |
| |
PKC inhibitors | Tamoxifen |
|
| x |
| ||
ssDNA scisson | Glycopeptides | Bleomycin |
|
| x |
| |
Glycopeptides | Bleomycin |
|
| x |
| ||
RNA synthesis | Amino-nucleoside | Puromycin |
| x | x | x | |
Amino-nucleoside | Puromycin |
| x | x | x | ||
Aziridines | Mitomycin C | x | x | x |
| ||
DNA structure through alkylation | Aziridines | Mitomycin C | x | x | x |
| |
Anthracyclines | Doxorubicin |
|
| x |
| ||
purine biosynthesis | DHFR inhibitors | Methotrexate |
|
| x |
|
Cell Wall Synthesis & Protein Synthesis
Cell Wall Synthesis, Cell Membrane Integrity & Protein Synthesis
Inhibit | Classification | Product Type | Product No. | Antibiotic Spectrum | |||
Gram -ve | Gram +ve | Fungi | Yeast | ||||
beta-lactamase, membrane phospholipids & polypeptide synthesis | Penicillins & polyenes | Antibiotic-Antimycotic solution | x | x | x | x |
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