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55819

Sigma-Aldrich

Atto 680-Biotin

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Synonym(s):

Biotin-Atto 680

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About This Item

MDL number:
MFCD04040686
UNSPSC Code:
12352200
PubChem Substance ID:
93577782
NACRES:
NA.32

product line

BioReagent

Assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

InChI

1S/C42H57N7O7S2/c1-4-48-18-10-12-27-20-30-35(22-33(27)48)56-36-23-34-29(21-31(36)45-30)28(26-58(53,54)55)24-42(2,3)49(34)19-11-15-39(51)44-17-9-5-8-16-43-38(50)14-7-6-13-37-40-32(25-57-37)46-41(52)47-40/h20-24,32,37,40H,4-19,25-26H2,1-3H3,(H4-,43,44,46,47,50,51,52,53,54,55)/t32-,37?,40-/m0/s1

InChI key

SMZVEGAKSTTZDO-QYIPWQSGSA-N

Application

Atto 680 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, good fluorescence quantum yield, excellent thermal and photo-stability, and very little triplet formation. Atto 680 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCBT5154
1462383

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Nicole Marmé et al.
Bioconjugate chemistry, 14(6), 1133-1139 (2003-11-20)
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655
Irene Drude et al.
Biochemical and biophysical research communications, 363(1), 24-29 (2007-09-11)
Over the past two decades, the structure and mechanism of catalytic RNA have been extensively studied; now ribozymes are understood well enough to turn them into useful tools. After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides
Slawomir Gwiazda et al.
Biochimie, 94(7), 1457-1463 (2012-04-03)
The RNA-world-theory is one possible explanation of how life on earth has evolved. In this context it is of high interest to search for molecular systems, capable of self-organization into structures with increasing complexity. We have engineered a simple catalytic
The design of a peptide linker group to enhance the SERS signal intensity of an atto680 dye-nanoparticle system.
Drake, P., et al.
Journal of Raman Spectroscopy, 41, 1248-1253 (2010)
Philip Tinnefeld et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 6(2), 217-222 (2005-03-09)
Light-harvesting complexes, one of nature's supreme examples of nanoscale engineering, have inspired researchers to construct molecular optical devices, such as photonic wires, which are optimised for efficient transfer of excited-state energy over large distances. The control parameters for the design
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