HLPPMAG-57K
MILLIPLEX® Human Liver Protein Magnetic Bead Panel - Metabolism Multiplex Assay
The Human Liver Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 9 liver protein biomarkers in human serum, plasma and tissue/cell culture samples.
About This Item
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 100.2%
(HGF)
accuracy: 93.7%
(ANGPTL3)
accuracy: 94.2%
(AFP)
accuracy: 96.6%
(FGF-23)
accuracy: 96.8%
(ANGPTL4)
accuracy: 98.8%
(FGF-21)
sensitivity: 0.004 ng/mL
(MinDC+2SD; FGF-21)
sensitivity: 0.014 ng/mL
(MinDC+2SD; FGF-23)
sensitivity: 0.018 ng/mL
(MinDC+2SD; HGF)
sensitivity: 0.054 ng/mL
(MinDC+2SD; FGF-19)
sensitivity: 0.144 ng/mL
(MinDC+2SD; AFP)
sensitivity: 0.218 ng/mL
(MinDC+2SD; FABP1)
sensitivity: 0.725 ng/mL
(MinDC+2SD; ANGPTL3)
sensitivity: 0.847 ng/mL
(MinDC+2SD; ANGPTL6)
sensitivity: 2.150 ng/mL
(MinDC+2SD; ANGPTL4)
standard curve range: 0.01-10 ng/mL
(FGF-21)
standard curve range: 0.04-30 ng/mL
(FGF-23)
standard curve range: 0.07-50 ng/mL
(HGF)
standard curve range: 0.14-100 ng/mL
(AFP)
standard curve range: 0.14-100 ng/mL
(FGF-19)
standard curve range: 0.27-200 ng/mL
(FABP1)
standard curve range: 0.69-500 ng/mL
(ANGPTL3)
standard curve range: 1.37-1000 ng/mL
(ANGPTL6/AGF)
standard curve range: 5.49-4000 ng/mL
(ANGPTL4)
inter-assay cv: <20%
intra-assay cv: <10%
(AFP)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL3)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL4)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL6/AGF)
inter-assay cv: <20%
intra-assay cv: <10%
(FABP1)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-19)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-21)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-23)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
MILLIPLEX® Human Liver Protein Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell culture samples: AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1/L-FABP, FGF-19, FGF-21, FGF-23 and HGF.
NOTE: When assaying ANGPTL6/AGF it is recommended that serum samples be used.
This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Specificity
The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Application
- Analytes: α-Fetoprotein (AFP), ANGPTL3, ANGPTL4, ANGPTL6 (AGF), FABP1 (L-FABP), FGF-19, FGF-21, FGF-23, HGF
- Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
- NOTE: Serum samples are recommended when measuring ANGPTL6/AGF
- Recommended Sample Dilution: 12.5 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be used undiluted or appropriately diluted in control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C is recommended for optimal sensitivity. 2 hours at room temperature (20-25° C) may also be used.
- Research Category: Metabolism
- Research Subcategory: Metabolic Disorders
Features and Benefits
Packaging
Storage and Stability
Other Notes
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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