HNS2MAG-95K
MILLIPLEX® Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay
The analytes available for this multiplex kit are: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, and TREM2.
About This Item
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species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 124%
(Neurogranin)
accuracy: 84%
(FABP3)
accuracy: 85%
(ApoE4)
accuracy: 94%
(TREM2)
accuracy: 97%
(Angiogenin)
sensitivity: 11.0 pg/mL
(MinDC + 2SD; TREM2)
sensitivity: 14.3 pg/mL
(MinDC + 2SD; FABP3)
sensitivity: 162.8 pg/mL
(MinDC + 2SD; ApoE4)
sensitivity: 19.9 pg/mL
(MinDC + 2SD; Neurogranin)
sensitivity: 5.8 pg/mL
(MinDC + 2SD; Angiogenin)
sensitivity: 6.1 pg/mL
(MinDC + 2SD; Ferritin)
standard curve range: 15-50,000 pg/mL
(TREM2)
standard curve range: 2-10,000 pg/mL
(Angiogenin)
standard curve range: 24-100,000 pg/mL
(FABP3)
standard curve range: 244-1,000,000 pg/mL
(ApoE4)
standard curve range: 5-20,000 pg/mL
(Neurogranin)
standard curve range: 6-25,000 pg/mL
(Ferritin)
inter-assay cv: <10%
intra-assay cv: <5%
(Angiogenin)
inter-assay cv: <10%
intra-assay cv: <5%
(FABP3)
inter-assay cv: <10%
intra-assay cv: <5%
(TREM2)
inter-assay cv: <15%
intra-assay cv: <10%
(Ferritin)
inter-assay cv: <15%
intra-assay cv: <10%
(Neurogranin)
inter-assay cv: <15%
intra-assay cv: <5%
(ApoE4)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
General description
MILLIPLEX® Human Neuroscience Bead Panel 2 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF), serum/plasma and cell/tissue culture supernatants for homogenates: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, TREM2.
Panel Type: Neuroscience
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. There is <5% cross-reactivity from ApoE2 and ApoE3 recombinant proteins on the ApoE4 beads.
Application
- Analytes: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, TREM2
- Recommended Sample type: Plasma, Serum, CSF, Cell/Tissue culture supernatant
- For Plasma/Serum a 1:10 dilution is recommended for the optimal detection of the analytes FABP3, Ferritin, Neurogranin and TREM2.
- For Plasma/Serum a 1:200 dilution for ApoE4 and Angiogenin is recommended.
- Because the sample dilutions differ for Plasma/Serum samples ApoE4 and Angiogenin cannot be plexed in the same assay as FABP3, Ferritin, Neurogranin and TREM2.
- CSF samples require a 1:10 dilution and so may all be plexed together
- Cell/Tissue culture supernatants or homogenates may be used neat or diluted in appropriate control medium.
- Bead diluent must be present in order to detect Angiogenin. (Biotinylated-Angiogenin detection antibody is included in the bead diluent.)
- Research Category: Neuroscience
Features and Benefits
Packaging
Storage and Stability
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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