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12041677001

Roche

CDP-Star®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

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About This Item

UNSPSC Code:
12352204

description

Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Quality Level

100

Assay

>98%

form

solution

packaging

bottle of 2 × 50 mL ([0.25 mM])

manufacturer/tradename

Roche

technique(s)

Northern blotting: suitable
Southern blotting: suitable
dot blot: suitable

storage temp.

2-8°C

Publishing

Purity: Purified by HPLC.

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
  • Southern blot
  • Northern blot
  • Dot blot
  • Colony and plaque hybridization
  • Gel shift assays
  • Chemiluminescent detection.

Features and Benefits

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution
CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
  • Save time with a fast, ultra-sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
Reuse the substrate by filtering and storing in sodium azide.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Other Notes

  • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
  • No preincubation step required.
  • Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
  • Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
  • Exposure times are shorter than with CSPD.
  • Do not use nitrocellulose membranes.
Repeat exposures can be made up to two days after the addition of substrate.
For life science research only. Not for use in diagnostic procedures.

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum).
Lin CY, et al.
Plant methods, 13(1), 113-113 (2017)
Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase.
Carrell ST, et al.
Nucleic Acids Research, 46(1), e1-e1 (2017)
Non-Radioactive Detection of Trinucleotide Repeat Size Variability.
Tome S, et al.
PLoS Currents, 6 (2014)
Ivano Legnini et al.
Nature methods, 16(9), 879-886 (2019-08-07)
Although messenger RNAs are key molecules for understanding life, until now, no method has existed to determine the full-length sequence of endogenous mRNAs including their poly(A) tails. Moreover, although non-A nucleotides can be incorporated in poly(A) tails, there also exists
Samuel Vega-Estévez et al.
mBio, 12(5), e0187121-e0187121 (2021-09-08)
Microorganisms need to adapt to environmental changes, and genome plasticity can lead to rapid adaptation to hostile environments by increasing genetic diversity. Here, we investigate genome plasticity in the CTG(Ser1) yeast Scheffersomyces stipitis, an organism with an enormous potential for
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