PIR00

Inhibitor Resistant Genotyping PCR ReadyMix^™

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

• Synonym(s): Inhibitor Resistant Genotyping PCR ReadyMix^™
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• feature: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart
• storage condition: protect from light
• technique(s): qPCR: suitable
• color: light blue
• input: crude DNA
• compatibility: Bio-Rad CFX384; Bio-Rad CFX96; Bio-Rad MJ Chromo4; Bio-Rad MJ Opticon 2 ; Bio-Rad MJ Opticon; Bio-Rad MiniOpticon; Cepheid SmartCycler; Eppendorf^® Mastercycler ep realplex ; Eppendorf^® Mastercycler ep realplex2 S; Illumina Eco qPCR; Qiagen Corbett Rotor-Gene 3000; Qiagen Corbett Rotor-Gene 6000; Qiagen Corbett Rotor-Gene Q; Roche LightCycler 480
• detection method: probe-based
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ does not contain an internal reference dye.
Inhibitor Resistant Genotyping PCR ReadyMix^™ has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite.

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required
• Compatible with both fast and conventional PCR cycling protocols

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Other Notes

Compatible instruments include:

• Bio-Rad^® CFX384^™
• Bio-Rad CFX96^™
• Bio-Rad MiniOpticon^™
• Bio-Rad/MJ Chromo4^™
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon^™
• Cepheid SmartCycler^®
• Eppendorf Mastercycler^® ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene^® 3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler^® 480

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

Eppendorf is a registered trademark of Eppendorf AG

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf AG

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

SmartCycler is a registered trademark of Cepheid, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable