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S7025

Sigma-Aldrich

50 bp DNA Step Ladder

for DNA electrophoresis

Synonym(s):

DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Quality Level

100

form

liquid

usage

100 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Components

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

Other Notes

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).
For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alper Dede et al.
Microbial pathogenesis, 143, 104134-104134 (2020-03-15)
Soil actinomycetes are a highly common group of bacteria and frequently studied as having secondary metabolites in the potential of producing the most preferred antagonistic content. Considering the continuous variation in soil structure, there is a potential for encountering different
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Laich F, et al.
International Journal of Systematic and Evolutionary Microbiology, 63, 3884-3891 (2013)

Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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