Skip to Content
Merck
  • Expression of SUMO enzymes is fiber type dependent in skeletal muscles and is dysregulated in muscle disuse.

Expression of SUMO enzymes is fiber type dependent in skeletal muscles and is dysregulated in muscle disuse.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020-01-08)
Arvind Venkat Namuduri, Gabriel Heras, Volker M Lauschke, Maurizio Vitadello, Leonardo Traini, Nicola Cacciani, Luisa Gorza, Stefano Gastaldello
ABSTRACT

SUMOylation is a dynamic, reversible, enzymatic drug-targetable post-translational modification (PTM) reaction where the Small Ubiquitin-like Modifier (SUMO) moieties are attached to proteins. This reaction regulates various biological functions like cell growth, differentiation, and it is crucial for maintaining organ homeostasis. However, the actions of SUMO in skeletal muscle pathophysiology are still not investigated. In this study, we quantified the abundance of the SUMO enzymes and determined the distribution of SUMOylated proteins along the fibers of nine different muscles. We find that skeletal muscles contain a distinctive group of SUMO enzymes and SUMOylated proteins in relation to their different metabolism, functions, and fiber type composition. In addition, before the activation of protein degradation pathways, this unique set is quickly altered in response to muscle sedentariness. Finally, we demonstrated that PAX6 acts as an upstream regulator of the SUMO conjugation reaction, which can become a potential therapeutic marker to prevent muscle diseases generated by inactivity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nitrotetrazolium Blue chloride, ≥90.0% (HPLC)
Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, pH 7, ≥98%
Sigma-Aldrich
Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
Sigma-Aldrich
Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt dihydrate, ACS reagent, 99.0-101.0%
Sigma-Aldrich
TWEEN® 20, for molecular biology, viscous liquid
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide, reduced disodium salt, ~98%, pkg of 10 mg (per vial)
Sigma-Aldrich
Sodium deoxycholate monohydrate, BioUltra, ≥99.0% (NT)
Sigma-Aldrich
Sodium deoxycholate monohydrate, BioXtra, ≥99.0% (titration)
Sigma-Aldrich
Iodoacetamide, BioUltra
Sigma-Aldrich
21-Hydroxyprogesterone, ≥97% (HPLC)