Skip to Content
Merck
  • DNMT1 reads heterochromatic H4K20me3 to reinforce LINE-1 DNA methylation.

DNMT1 reads heterochromatic H4K20me3 to reinforce LINE-1 DNA methylation.

Nature communications (2021-05-05)
Wendan Ren, Huitao Fan, Sara A Grimm, Jae Jin Kim, Linhui Li, Yiran Guo, Christopher James Petell, Xiao-Feng Tan, Zhi-Min Zhang, John P Coan, Jiekai Yin, Dae In Kim, Linfeng Gao, Ling Cai, Nelli Khudaverdyan, Burak Çetin, Dinshaw J Patel, Yinsheng Wang, Qiang Cui, Brian D Strahl, Or Gozani, Kyle M Miller, Seán E O'Leary, Paul A Wade, Gang Greg Wang, Jikui Song
ABSTRACT

DNA methylation and trimethylated histone H4 Lysine 20 (H4K20me3) constitute two important heterochromatin-enriched marks that frequently cooperate in silencing repetitive elements of the mammalian genome. However, it remains elusive how these two chromatin modifications crosstalk. Here, we report that DNA methyltransferase 1 (DNMT1) specifically 'recognizes' H4K20me3 via its first bromo-adjacent-homology domain (DNMT1BAH1). Engagement of DNMT1BAH1-H4K20me3 ensures heterochromatin targeting of DNMT1 and DNA methylation at LINE-1 retrotransposons, and cooperates with the previously reported readout of histone H3 tail modifications (i.e., H3K9me3 and H3 ubiquitylation) by the RFTS domain to allosterically regulate DNMT1's activity. Interplay between RFTS and BAH1 domains of DNMT1 profoundly impacts DNA methylation at both global and focal levels and genomic resistance to radiation-induced damage. Together, our study establishes a direct link between H4K20me3 and DNA methylation, providing a mechanism in which multivalent recognition of repressive histone modifications by DNMT1 ensures appropriate DNA methylation patterning and genomic stability.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Thymidine, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
A-196, ≥98% (HPLC)
SKU
Pack Size
Availability
Price
Quantity