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Mutational effects depend on ploidy level: all else is not equal.

Biology letters (2012-10-12)
Aleeza C Gerstein
ABSTRACT

Ploidy is predicted to influence adaptation directly, yet whether single mutations behave the same in different ploidy backgrounds has not been well studied. It has often been assumed theoretically that aside from dominance, selective parameters do not differ between cells of varying ploidy. Using the budding yeast Saccharomyces cerevisiae, I compared the effect size of 20 adaptive mutations in haploids and homozygous diploids and found, surprisingly, that the same mutations often had a much larger effect in haploids than homozygous diploids. This empirical result demonstrates that it cannot be assumed that mutations will have the same effect in haploids and homozygous diploids.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nystatin Suspension, suspension, 10,000 unit/mL in DPBS, BioReagent, suitable for cell culture
Nystatin, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Nystatin, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Nystatin, powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Nystatin, ≥4,400 USP units/mg